CACTUS GRANDIFLORUS, A HOMEOPATHIC PREPARATION HAS PROTECTIVE EFFECT AGAINST DOXORUBICIN INDUCED CARDIOMYOPATHY IN RATS

ABSTRACT

To evaluate the cardioprotective action of Cactus grandiflorus in doxorubicin‑induced cardiomyopathy in rats on the basis of biochemical and histopathological analysis and compare the cardioprotective potential of Cactus grandiflorus with the standard drug Vitamin E with drug toxicity on liver and kidney by means of blood parameters. Clinical use of doxorubicin is limited due to its dose dependent cardiotoxicity inspite of its broad spectrum activity against variety of malignancies and solid tumors. Doxorubicin induced acute cardiotoxicity may be caused with the initiation of chemotherapy whereas chronic toxicity develop generally after completion of therapy and occurs in patients after cumulative dose of ≥500 mg doxorubicin/m. This study was aimed to investigate the alcoholic extract (mother tincture) of Cactus grandiflorus on doxorubicin induced cardiomyopathy. Male wistar albino rats were used in vivo model for the study of seven days. Two different doses (0.5 mL and 1.1 mL) of Cactus grandiflorus mother tincture were given orally for seven days in this post treatment study. doxorubicin 15 mg/kg was given ip on the first day. There was significant decrease in level of antioxidant enzymes like glutathione superoxide dismutase (SOD) and catalase (CAT) in animals treated with DOX 15 mg/kg and elevated level of lactate dehydrogenase (LDH), serum glutamate oxaloacetatae transaminase (SGOT), and serum glutamate pyruvate transaminase (SGPT) alkaline phosphates and serum creatinine. Post treatment with Cactus grandiflorus resulted in increase in level of antioxidant enzymes and decrease in level of LDH, SGOT, SGPT alkaline phosphates and serum creatinine. Histological investigation had shown that doxorubicin‑treated group had scattered myocardial cells with multiple vacuolar myopathic changes. The samples from the groups treated with 0.5 mL and 1.1 mL Cactus grandiflorus with doxorubicin showed a significant reduction in the number of vacuolated cells, s of sample from the saline Cactus grandiflorus only and vitamin E only groups showed normal myocardium.cheap trx canada for sale  These results suggested mother tincture of Cactus grandiflorus had protective effect against doxorubicin‑induced cardiotoxicity and had potential as a cardioprotective agent.

Key words: Cactus grandiflorus, Doxorubicin, Homeopathic preparation, Cardiotoxicity.