SCREENING THE LEAF EXTRACT OF PERGULARIA DAEMIA (FORSSK.) CHIOV., FOR ITS ANTIOXIDANT PROPERTY AND POTENTIAL ANTIMICROBIAL ACTIVITIES ON MEDICINALLY IMPORTANT PATHOGENS

ABSTRACT

Pergularia daemia (Forssk.) chivo., is a shrub of Asclepiadaceae family found in the plains throughout India and in tropical countries worldwide. The present study aims at comparative analysis of qualitative and quantitative secondary metabolite components and to determine the biological activities of the leaf by extracting the methanolic soluble components of Pergularia daemia in order to establish a theoretical relationship between its constituents, biological activity and use. Pergularia daemia showed its anti-bacterial activity on Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, trx canada Pseudomonas aeruginosa and Escherichia coli and among fungi, Candida albicans and Candida glabrata. Ampicillin and nystatin are used as standards. The extract is having more activity on Escherichia coli and Candida glabrata. The MIC and MBC values for the extract are determined. MIC and MBC values for Bacillus subtilis, Bacillus cereus, and Escherichia coli are found to be lowest ie.,15.62/31.25 and 7.81/15.62 and Candida glabrata it was found to be 31.25/125. In case of staphylococcus and pseudomonas species the concentration is 62.5/>500 and 62.5/125. These activities are found to be due to phenols and flavonoids present in the extract.

Key words: Pergularia daemia, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC).