AN IN VIVO AND IN VITRO STUDIES ON THE ANTIOXIDANT PROPERTY OF EPIGALLOCATECHIN GALLATE ON SODIUM FLUORIDE INDUCED TOXICITY IN RATS

ABSTRACT

In the present study was designed to explore the protective effect of EGCG against NaF induced changes in plasma lipid peroxidation and antioxidant status such as Vitamin C , E, and reduced glutathione and In vitro free radical scavenging assays of EGCG. Administration of NaF (25 mg/kg bw) orally for 4 weeks significantly (p<0.05) elevated the levels of lipid peroxidation and decreased the levels of non-enzymatic antioxidants like GSH, Vit.C and E in the plasma of rats. Administration of EGCG revealed a significant (p<0.05) decrease in lipid peroxidation with a significant (p<0.05) increase in the antioxidant status in the plasma of fluoride treated rats. For in vitro scavenging effect of EGCG, five different concentrations (10, 20, 30, 40, and 50 µM) were used. Free radical scavenging activity was evaluated by following invitro assays such as 1,1-diphenyl-2- picrylhydrazyl (DPPH•), 2,2-azinobis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS), superoxide anion (O•), hydroxyl (OH•) radicals, reducing power assay, total antioxidant assay, metal chelating assay and nitric oxide (NO) assay. In addition to that, ascorbic acid and butylated hydroxyl toluene was used as the standard antioxidants for free radical scavenging assays. The results confirmed that, the free radical scavenging and antioxidant activity of EGCG has increasing with increasing concentrations. Interestingly, among the different concentrations tested, 40 μM of EGCG showed the highest antioxidant and free radical scavenging activities in almost all the in vitro assays. Hence the present study proved that the EGCG has potent In vitro and in vivo antioxidant activity which augmenting its therapeutic potential against fluoride induced toxicity.

Key words: EGCG, Antioxidant, in vivo, in vitro, NaF, Rat.