PHARMACOLOGICAL EVALUATION OF HERBAL EXTRACTS FOR THEIR INVITRO HYPOGLYCEMIC ACTIVITY
 

ABSTRACT

Diabetes mellitus is a metabolic disorder and the management is an important criterion for pharmacotherapy.  The medicinal plants play very important role in preventing the progress of the disease. Present study deals with screening of polyherbal extracts using Invitro techniques for its antidiabetic activity. The plants used in the study are Eugenia jambolana and Cinnamomum zeylenicum. The plants were extracted using direct soxhlet method with various solvents.  The polyherbal extracts were subjected to invitro evaluation using cell culture assays, alpha-glucosidase inhibition and antioxidant property. The cell lines 3T3L1 C2C12 and HepG2 were used to measure the uptake of glucose. The results showed that the glucose uptake was more in APKJ-004 polyherbal extract when compared with other extracts. α-glucosidase inhibitory activity was found in all the extracts and they were comparable to the standard acarbose and the highest inhibitory activity was found in the polyherbal extract APKJ004 (IC₅₀147.2µg/ml). The invitro antioxidant potential revealed that the extract APKJ004 is more potent and is comparable to the standard. Based on the invitro evaluations<a href="http://www.aucheaptrx.com/">cheap trx for sale</a>  the unique combination of glucose uptake activity and α glucosidase inhibition potential of extract APKJ-004 with prominent hypoglycemic activity will aid in the treatment of diabetis mellitus. The invivo experiments to prove the results obtained in invitro studies are in progress.

 Key words: Antidiabetic activity, 3t3-L1, C2C12, HepG2, polyherbal extract, Glucose uptake, α-glucosidase.