EVALUATION OF ANTI-INFLAMMATORY POTENTIAL OF PETAL EXTRACTS OF CROCUS SATIVUS “CASHMERIANUS”

ABSTRACT

Different petal extracts of Crocus sativus “Cashmerianus” were examined for anti-inflammatory activity by in-vitro human red blood cell (HRBC) membrane stabilization method and in-vivo carrangeenan- induced rat paw edema. The petroleum ether, chloroform, ethyl acetate, methanol and aqueous extracts of petals showed 49.36%, 59.39%, 48.10%, 70.37% and 68.89% protection at the dose of 400mg/ml respectively by in-vitro method. However, in-vitro most promising effective chloroform, methanol and aqueous extracts were selected for in-vivo anti-inflammatory method. The in-vivo anti-inflammatory activity was found as 50%, 63.16% and 57.89% inhibition of paw volume after 5h of chloroform, methanol and aqueous extracts treated at the dose of 400mg/kg groups respectively. <a href="http://www.aucheaptrx.com/">cheap trx for sale</a> The potency of the petals extracts were compared with Diclofenac (10 mg/ml) for in-vitro and 10mg/kg for in-vivo model. The methanol and aqueous extract showed the most promising anti-inflammatory activity in membrane stabilizing action on human red blood cell membrane and reduction of edema in carrageenan induced rat paw edema model. The results of present and first time reporting this study on petals extracts of Crocus sativus “Cashmerianus” demonstrate that extracts of  petals possess significant (p<0.05) anti-inflammatory potential.

 Keywords: Crocus sativus, anti-inflammatory, carrageenan, Human red blood cell membrane stabilization method .