HPTLC DETECTION OF POLYPHENOLS AND FLAVONOIDS OF CAREYA ARBOREA LEAVES AND STUDY OF ANTIMICROBIAL EFFECT 

ABSTRACT

Careya arborea  belongs to the family Lecythidaceae, is wellknown as Kumbhi in Ayurveda and has been employed  in Ayurvedic treatment. A countable studies showed that the methanolic extract of stem bark of this plant exhibited antioxidant and antimicrobial effect.As in medicinal herbs the secondary metabolites are mainly polyphenols and flavonoids and their quality and quantity depends on parts of the plant. Considering that the leaves of this palnt could contain some polyphenols and flavonoids that may have different pharmacological properties, we have extracted the dry leaves using different individual and mixture of organic solvents such as water, acetone, methanol, methanol + water <a href="http://www.aucheaptrx.com/">cheap trx for sale</a> and ethyl acetate  + methanol + water. HPTLC analysis showed that leaves extracted by ethyl acetate + methanol + water contains the highest percent of plolyphenols than by methanol. Such leaf extract of c.arborea exhibited a significant antimicrobial effect in disc diffusion test against bacterial culture under test.

Key-words: HPTLC analysis, careya arborea, leaves, polyphenols, antimicrobial effect.