HEPATOPROTECTIVE POTENTIAL OF LAWSONIA INERMIS L. (SEEDS) 

ABSTRACT

This study aimed to assess the hepatoprotective activity of ethanol (90%) extract and its ethyl acetate fraction Lawsonia inermis L. seeds on carbon tetrachloride (CCl₄) induced hepatotoxicity in rats and to ascertain the claim of its use in liver disorders. Pre-treatment of rats with doses of 200 and 400 mg/kg, b.wt; p.o. of the ethanol extract and its ethyl acetate fraction significantly (P < 0.05) lowered serum transaminases (AST and ALT), alkaline phosphatase (ALP), total bilirubin (TB) and increased the levels of the total proteins (TP) and albumin (ALB) respectively, in a dose dependent manner against the significant (P < 0.01) alteration of these damaged marker enzymes when challenged with CCl₄ (0.5 ml/kg, i.p.). Parallel to these changes, the seeds extract and its fraction prevented CCl₄ induced oxidative stress by significantly restoring the levels of reduced glutathione and lowering the levels of hepatic malondialdehyde by inhibiting the production of free radicals. These biochemical parameters were supplemented by histopathological examination of liver sections. cheap trx for sale Taken collectively, these findings suggest that ethyl acetate fraction revel more significant (P < 0.05) hepatoprotective potential against CCl₄ induced hepatotoxicity in rats and confirms the folklore use of this plant.

Key words: Carbon tetrachloride; Hepatoprotective; Lawsonia inermis; Oxidative stress; Transaminases.