ANTIOXIDANT ACTIVITY OF SPILANTHES ACMELLA EXTRACTS 

ABSTRACT

Many medicinal plants were with a long history of use in folk medicine against a variety of diseases. Recently, many researchers have taken a great interest on medicinal plants for their phytochemical constituents and biological activities including antioxidant activity. The present study was aimed to evaluate phytochemical analysis, quantification of total phenolic, alkaloid contents and antioxidant activity of Spilanthes acmella extracts (70% Ethanol, Methanol, Ethyl Acetate and Hexane). The total phenolic and alkaloid contents were quantified by using gallic acid and atropine are as standards and antioxidant activity was evaluated by using three free radicals (Superoxide, Hydroxyl and DPPH). Spilanthes acmella extracts revealed the presence of different phytochemical constituents like steroids, terpenoids, flavanoids, alkaloids, glycosides, tannins, carbohydrates, oils and amino acids. The methanol extract have more phenolic and alkaloid contents i.e. 38.83±0.68 (mg/gm) and 26.37±0.16 (mg/gm) than other extracts. The extracts were produced concentration dependent percentage inhibition on free radicals and produced maximum activity at concentrations of 320 and 640µg. Among all extracts, methanolic extract showed better activity compared to other extracts with mean IC₅₀ values on superoxide, hydroxyl and DPPH radicals were 189µg, 153µg and 313µg. The above results suggest that Spilanthes acmella extracts have antioxidant activity. cheap trx canada for sale  Further research is in progress i.e. to isolation and characterization of active molecules (compounds) responsible for the antioxidant activity which can be used to treat various diseases.

Key words: Spilanthes acmella, Aerial parts, Total Phenolic content, Total alkaloid content, Free radicals, Antioxidant Activity.