FREE REDICALS SCAVENGING ACTIVITY OF POLYHERBAL FORMULATION (DIASWETA)

 ABSTRACT

Many oxidative stress related diseases are as a result of accumulation of free radicals in the body. A lot of researches are going on worldwide directed towards finding natural antioxidants of plants origins. Diasweta a polyherbal formulation have been used as a healthy food and traditional drug in India. The present study investigated the phenolic compounds and antioxidant activity of ethanolic extract from Polyherbal formulation Diasweta. The antioxidant activity was measured by Reducing power, Hydrogen peroxide and Nitric oxide inhibition activities. Total phenol content was determined as Gallic acid equivalents by the Folin-Phenol method. Diasweta exhibited its scavenging effect in concentration dependent manner on nitric oxide radical, hydrogen peroxide, and reducing power. The total phenolic content was higher in the ethanol fraction (57.47 ± 1.74 g GAE). The ethanol extract (OD₇₀₀ = 1.96) also exhibited the strongest reducing power. Our findings provide evidence that the crude ethanolic extract of Diasweta is a potential source of natural antioxidants.

Key words: Reducing power assay, Hydrogen peroxide assay, Antioxidant activity, Free radicals scavenging.