SYNTHESIS, CHARACTERIZATION, ANTIOXIDANT, DNA CLEAVAGE AND CYTOTOXIC STUDIES OF AMINO DERIVATIVES OF LAWSONE 

ABSTRACT

2-Hydroxy-1,4-naphthaquinone (Lawsone)  was isolated from the leaves of   lawsonia inermis. Two amino derivatives, 2-[(Para –methyl) anilino]-1, 4-naphthoquinone (PAN) and 2-(thiol anilino)-1, 4- naphthoquinone (TAN) were synthesized from lawsone by ultrasound accelerated technique, a new method which have not been so far reported.  These compounds were characterized using various spectral techniques and elemental analysis.  Its fluorescence emission wavelength was measured in acetonitrile solution. The   synthesized   compounds were screened for their antioxidant activity. The in vitro cytotoxic effect was studied by using trypan blue dye exclusion method. DNA Cleavage activity of the compounds was also investigated on pET20b plasmid DNA by gel cheap trx canada for sale  electrophoresis experiments. These derivatives exhibited significant activity in all the studies conducted. In addition, the fluorescent property of the compounds may be useful as molecular fluorescent probes for antibody- antigen biochemical species.

Key words: Lawsone, antioxidant study, DNA cleavage, and cytotoxic activity.