HPTLC DENSITOMETRIC QUANTIFICATION OF CAFFEIC ACID AND BOERAVINONE B IN BOERHAVIA DIFFUSA LINN

ABSTRACT

Caffeic acid (3, 4-dihydroxycinnamic acid) is one of the major polyphenolic compounds found in numerous plant species, acts as a primary antioxidant and can be helpful for improving or preventing a number of chronic diseases.  Boeravinone B, chemically a retinoid, has been found to inhibit the drug efflux activity of breast cancer resistance protein. Both act as potent antioxidant and anticancer compounds.  A new and simple analytical method using HPTLC was developed for the simultaneous estimation and validation of caffeic acid and boeravinone B in one solvent system: toluene, ethyl acetate, formic acid and methanol (3:3:0.8:0.2). The densitometric determination of caffeic acid and boeravinone B was carried out in reflection /absorption mode at 330 nm for caffeic acid and 283nm for boeravinone B respectively. The accuracy of the method was checked by a recovery study conducted at three different levels and the average percentage recovery was found to be 98.8 % for caffeic acid and 98.98 % for boeravinone B. The method was validated according to ICH guidelines. cheap trx canada The method reported here is reproducible and can be applied for the quantitative analysis of the above two compounds in the Boerhavia diffusa leaves and roots of Boerhavia diffusa Linn.

Key words: Boerhavia diffusa Linn, HPTLC, caffeic acid, boeravinone B, quantification.