ANTI-DIABETIC ACTIVITY OF GLOCHIDION VELUTINUM L. EXTRACT IN ALLOXAN - INDUCED TYPE-II DIABETIC RATS

ABSTRACT

The current study   was done to investigate the anti-diabetic activity of the leaves of Glochidion velutinum  in vivo by using alloxan induced  diabetes model in rats. We want to know its safety and efficacy by local herbalists for the treatment of diabetes mellitus. Active principle is responsible for the therapeutic effect, preliminary phytochemical analysis of the aqueous and methanolic extracts of the plant has been investigated. Crude aqueous (AQ) and methanol (i.e., MEOH) extracts of the leaves of Glochidion velutinum were administered to both normal and alloxan induced diabetic male albino rats (Wistar strain). The blood glucose levels were measured at 0, 2, 4, 6 and 8 h each day up to 15 days after oral administration of AQ and MEOH extracts. We observed that significant reduction in blood glucose levels in treated and standard Glibenclamide (0.28 mg/kg) group without causing any hypoglycemic effect compared to normal rats. The statistical significance was evaluated by using dunnet multiple comparison test (p<0.05 is significant). Both polar and methanolic extracts of the leaves of Glochidion velutinum exhibited significant anti-hyperglycemic activity. Observable parameters in ethanolic extract and aqueous extract of G. velutinum (400mg/kg) shows a significant decrease of blood glucose levels i.e., 169±3.05, 165.83±2.98 compared with the positive control 314±05 at various time intervals.  This study provides scientific evidence that the leaves of Glochidion velutinum have anti-diabetic efficacy and further investigation is need to be conducted for the structural elucidation and isolation of active principle responsible for antidiabetic activity.

Key words: Golchidion velutinum, Diabetic, Glibenclamide.