CEREBRO PROTECTIVE EFFECT OF FLAVONOID OF EVOLVULUS ALSINOIDES IN BILATERAL COMMON CAROTID ARTERY (BCCAO) INDUCED CEREBRAL ISCHEMIA

ABSTRACT

                The  present  study  was  conducted  to  evaluate  the  cerebro  protective  activity  of   the flavonoid  of   methanolic  leaf  extract  of Evolvulus alsinoides against  cerebral  ischemic reperfusion – induced  cerebral  infarction  in  rats.  The leaves of Evolvulus alsinoides were extracted using soxhelet extraction. The extract was subjected to column chromatograph. The chemical tests were carried out toconform the flavonoid. The structural elucidation was done by different spectral analysis. This pure compound was used for further studies. Male Wistar rats weighing 250-280gms were used in the present study. The animals were anesthetized with thiopentone sodium (45mg/kg) by i.p. earlier published method was used for induction of ischemia followed by ketamine (100mg/kg) i.p which is skeletal muscle relaxant. Prior to the incision Lidocain administrated (4mg/kg) subcutaneously. Ischemic condition was achieved by BCCAO for 45 min, followed by a reperfusion period of 48h. The Allopurinol (10 mg/kg) (XO inhibitor), L-NAME (10 mg/kg) (NO inhibitor) and Nimesulide (20 mg/kg) (COX inhibitor) were administered 10min before ischemia and EA (200mg/kg) was administered for 7 days p.o. After the reperfusion period the animals were sacrificed immediately and the brain was decapitated, homogenized, supernatant was collected then when treated various enzymatic estimations were carried out. Histopathological examination showed that Evolvulus alsinoides conformed the cerebro protective effect. The biochemical estimation showed that there was a significant decrease in the oxidant levels of MDA and MPO and significant increase in the antioxidant levels of CAT and SOD. The Flavonoid of Evolvulus alsinoides significantly inhibit the percentage of damage to the brain. Therefore the present study exhibited cerebroprotective effect when administered alone. A synergistic effect was observed when Evolvulus alsinoides was administered along with Nimesulide.

Key words: Cerebral ischemia, Flavonoid,   Evolvulus alsinoides,  BCCAO,  Oxidative stress,  Reperfusion injury.