EVALUATION OF ANTI-PYRETIC AND ANTIMICROBIAL POTENTIALS OF SIDA SPINOSA LINN. ETHANOLIC ROOT EXTRACT IN RATS WITH POSSIBLE MECHANISM

ABSTRACT

                Study was conducted to understand the Antipyretic and antimicrobial potential and mechanism of action of Sida spinosa Linn. Ethanolic root extract in experimental animals. Roots were extracted with ethanol by successive extraction in soxhlet apparatus. The doses of the extract selected were 200 and 400 mg/kg b.w. Antipyretic potential was evaluated in Brewer’s yeast and 2,4-dinitrophenol induced pyrexia in rats along with Antimicrobial activity by agar well diffusion technique, MIC and MBC. Ethanolic extract (400mg/kg b.w) demonstrated highly significant (P<0.01) antipyretic activity when challenged in both yeast and 2,4-dinitrophenol induced pyrexia. Maximum attenuation in yeast and 2,4-dinitrophenol induced pyrexia were 74.12 % and 65.73% at 3h. Antimicrobial activity against S. aureus, B. subtilis, E. coli and S. aeruginosa, was carried out by disc diffusion and micro dilution methods. All microbes were sensitive and activity was concentration dependent. Sida spinosa root extract shown potent antipyretic activity in yeast and 2,4-dinitrophenol induced pyrexia, supported by its antimicrobial potential which is comparable with standard aspirin & has therapeutic potential.

Key words: Sida spinosa, Antipyretic & Antimicrobial activity, Minimum inhibitory concentration (MIC), Minimum bacterial concentration (MBC), Aspirin, Tetracycline.