EVALUATION OF ANTIULCER AND IN-VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF PSIDIUM GUAJAVA ROOT IN ALBINO WISTAR RATS

ABSTRACT

The study was carried out to find out the antiulcer activity of methanolic extract of Psidium guajava. For this study albino wistar rats(150-200g) were used .Four groups  of rats were selected containing six animals in each group .The study was carried on different gastric ulcer models and ulcers were induced by pyloric ligation and aspirin .The animals were treated with Omeprazole 20mg/kg and MEPG 200 and 400mg/kg. Ulcer index was calculated in both models .In pyloric ligation model, free acid, total acid, total protein and mucin contents were also determined. In in-vitro antioxidant, Free radical scavenging (DPPH assay) activity and lipid peroxidation content were estimated. MEPG produced significant reduction in ulcer index (1.5) when compared with control (5.83) in both ulcer models .In pylorus ligated model it showed significant reduction in volume of acid secretion and increased mucin and total protein content .It also produced significant increase  in Free radical scavenging and lipid peroxidation content. MEPG produces significant anti-ulcer activity which is comparable to standard drug Omeprazole. It also produces significant anti-oxidant activity which is comparable to standard ascorbic acid.

Key words: Pyloric ligation, DPPH, Lipid peroxidation, MEPG-Methanolic extract of Psidium guajava.