IN-VITRO FREE RADICAL SCAVENGING POTENTIAL OF POLY HERBAL EXTRACT

ABSTRACT

The study was designed to examine the in-vitro free radical scavenging potential of ethanolic polyherbal extract(EPHE).The antioxidant activity was evaluated by DPPH(1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity, superoxide, hydroxyl radical, hydrogen peroxide and ferric reducing assay with reference standard  Quercetin. The EPHE was found to more effective in the DPPH radical scavenging activity. The IC₅₀ of EPHE and Quercetin were found to be 20.1μg/ml and 0.455μg/ml respectively. An IC₅₀ value was found that EPHE of 83.61μg/ml effective in scavenging superoxide radical when compare with Quercetin (standard) 49.641μg/ml. The IC₅₀ value was found to be 9.745µg/ml, 24.41µg/ml quercetin and EPHE respectively. Hence the EPHE can be considered as a good scavenger of hrdroxyl radicals. The FRAP values for the EPHE compared with Quercentin that have significant reducing power. Scavenging activity of hydrogen peroxide in 54.67µg/ml,121.8 µg/ml quercetin and EPHE respectively as reference compound was not remarkably different. It is concluded that the EPHE which exhibits high antioxidant and free radical scavenging activities.

Key words: In-vitro, Free radical scavenging, Poly herbal extract.