EVALUATION OF WOUND HEALING ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES FROM AQUEOUS EXTRACT OF CASSIA AURICULATA L.

ABSTRACT

In this present study, biosynthesis of AgNp’s from aqueous extracts of C. auriculata and its wound healing activity was documented. The synthesis of AgNp’s was achieved by treating AgNo₃ solution with aqueous extract of C. auriculata. The formations of AgNp’s were confirmed by a color change of the solution from watery to brown color. The active phytochemicals present in the plant was responsible for the immediate reduction of silver ion (Ag+) to metallic silver nanoparticles (Ag^o). The reduced AgNp’s were characterized by Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM), FTIR, UV–vis spectroscopy. The spherical shaped AgNp’s were observed and it was found to be 15-90 nm range of sizes. The particle size range of AgNp’s monitored by particle size analyzer Mastsizer 2000 and result was found to be 0. 257μm. The wound healing activity of biosynthesized AgNp’s was studied by excision and incision wound models. In the excision wound model the AgNp’s showed increased the percentage of wound contraction than standard drug Povidone iodine ointment and aqueous extracts of C. auriculata  treated group of animals. For incision wound model AgNp’s showed maximum rate of tensile strength when compared to standard drug and aqueous extract of C.auriculata treated group of animals. The results suggest that biosynthesized AgNp’s from aqueous extracts of C. auriculata posses a significant wound healing potential in normal wound.

Key words: Green synthesis, silver nanoparticles, Characterization, UV-Vis, TEM, SEM.