IN VITRO ANTI CANCER ACTIVITY OF VARIOUS FRACTIONS OF HYDRO ALCOHOLIC EXTRACT OF DALBERGIA LATIFOLIA ROXB.

Dalbergia latifolia Roxb. (DLR) plant is majorly available in India, traditionally used for various diseases. Hence the aim of the present study is to compare the phytochemical investigation and in vitro anticancer activity of various fractions of hydro alcoholic extract of Dalbergia latifolia Roxb. against L6, EAC, MCF 7, HEP G2 and Hela cell lines. MTT assay was used to assess the in vitro anti cancer activity. The extract at various doses were treated against all the cell lines. The parameters analyzed were percentage of cytotoxicity, percentage of cell viability and IC50 values were evaluated. In results the methanolic fraction of DLR showed more potent activity than the all other fractions. All the fractions does not show any cytotoxicity against the normal L6 cell lines. nHexane, chloroform fractions showed very minimal activity, the ethyl acetate fraction showed moderate activity against all the cell lines. The Methanol fraction showed significant activity against all cell lines, especially MCF 7 cell lines were more susceptible to methanol fraction. The anti cancer nature of the methanol fraction is due to the presence of phytoconstituents like flavanoid, alkaloid, triterpene etc.