PHYTOCHEMICAL SCREENING AND IN VITRO ANTICANCER EFFECT OF EXTRACTS ENTANDROPHRAGMA ANGOLENSE A MEDICINAL PLANT USED IN THE TREATMENT OF OBSTETRIC FISTULA IN IVORY COAST

The study focused on extracts of Entandrophragma angolense a plant of Meliaceae family used for Ivorian traditional healers to treat obstetric fistula. The investigation was carried to evaluate in vitro anticancer effect of extracts aqueous and ethanol of Entandrophragma angolense bark on the murin mastocytoma cancer cell line (P815) and the phytochemical screening of the extract as well. In vitro anticancer effect was evaluated by the cellular cytotoxicity against the murin mastocytoma cell line (P815). Cellular cytotoxicity was determined by the MTT assayand the phytochemical screening was based on differential staining and precipitation reactions. The in vitro anticancer effect showed a dose dependant cytotoxic effect. It was observed that the ethanolic extract has the best cytotoxic effect with an IC50 of 44.30 ?g/mL against 2.5 ?g/mL for methotrexate. While the aqueous extract has a weak cytotoxic effect with an IC50 of 200 ?g/mL. Phytochemical screening of extracts shows the presence of alkaloids, flavonoids (flavones, flavonol), tannins, coumarins, sterol, triterpenoids and saponins. Our results suggest that extracts of E. angolense bark contains several chemical groups and possess a best in vitro anticancer effect with the ethanolic extract and a weak effect with aqueous extract against P815 tumor cell line.