STUDY OF IN VITRO ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTICANCER POTENTIAL OF BLUMEA OXYODONTA DC.

The present study aimed to establish scientific authenticity anticancer, antioxidant and anti-inflammatory activities of ethanol and aqueous extracts of the whole plant Blumeaoxyodonta DC. The antioxidant activity of the extracts were assessed by DPPH radical scavenging assay, ferric reducing power assay and hydrogen peroxide radical scavenging assay using ascorbic acid as the standard. Anti-inflammatory activity of the extracts was evaluated using HRBC membrane stabilization method where diclofenac sodium was used as the standard. The results indicated that the ethanolic and aqueous extracts have significant antioxidant, anti-inflammatory and cytotoxicity activity in a dose-dependent manner. The extract demonstrated potent scavenging of free radicals such as hydrogen peroxide and superoxide and possessed high reducing power against the standard ascorbic acid. Potent inhibition of HRBC membrane lysis and protein denaturation proved its antiinflammatory activity when compared to standard diclofenac sodium. Brine shrimp lethality test provided strong results for the anticancer activity which was confirmed by the MTT assay performed on cell lines. The finding powerfully supports that the ethanolic extract of the plant BlumeaoxyodontaDC could be used as a source of anticancer drug attributed to its phenolic content that makes it prompting for further isolation of bioactive compounds