ANTIDIABETIC, ANTIOXIDANT, ANTIBACTERIAL ACTIVITIES AND GC-MS ANALYSIS OF WITHANIA COAGULANS DUNAL- (RISHYAGANDHA)

Withania coagulans (Indian rennet) is an important medicinal plant which belongs to family Solanaceae. Roots, leaves, stem, green berries, fruits, seed, bark of this plant is used as folk medicine, because of its medicinal properties like antifungal, antibacterial, antitumor, antidiabetic, antibiotic, antistress, antiasthmatic, anticancer, antioxidant, antiinflammatory activities. The research studies were carried out to evaluate the antidiabetic, antioxidant, and antibacterial activities of dried fruit extract of W. coagulans and to identify the bioactive compounds by GC-MS analysis. Antioxidant activities such as DPPHradical, superoxide (O2 .-) radical scavenging activities, Fe3+ reducing power and phosphomolybdenum reduction activities were performed for dried fruit extract in order to reveal the efficacy of antioxidant molecules in neutralizing the free radicals. The maximum alpha amylase enzyme inhibition ofmethanol dried fruit extract of W. coagulans was 66.60±0.23% at 300 ?g/mL concentration and the IC50 value was 174.91?g/mL concentration respectively. The maximum DPPH? radical and superoxide (O2 .-) radical scavenging activities of methanol dried fruit extract of W. coagulans were 54.68±0.23% and 42.85±0.35% at 120 ?g/mL concentration and the IC50 values were 118.53 ?g/mL and 140.02 ?g/mL concentration respectively. The maximum Fe3+ and Mo6+ reduction of methanol dried fruit extract of W. coagulans were 77.94±0.45% and 86.13±0.21% at 120 ?g/mL concentration and the RC50 values were 40?g/mL and 17.07?g/mL concentration respectively. GC-MS analysis of methanol dried fruit extract of W. coagulans showed the presence of many active compounds such as a-Pinene, Longifolene-I2.Elaidic acid, isopropyl ester, etc. The antibacterial activity for the methanol dried fruit extract of W. coagulans confirmed maximum inhibitory property as 20 mm against tested bacterial pathogens at 375 ?g/mL concentration.