EVALUATION OF ANTI INFLAMMATORY AND ANTI PROLIFERATIVE PROPERTIES OF HAEMOLYMPH OF SCYLLA SERRATA (FORSSKAL, 1775)

Marine organisms comprise approximately half of the total biodiversity on the earth and the marine ecosystem is the greatest source to discover useful therapeutics. The potential of marine crabs as source of biologically active products is largely unexplored. The present study was aimed to explore the in vitro ant proliferative and anti-inflammatory activities of haemolymph of Scylla serrata.(Forsskal,1775). Preliminary zoo chemical screening of the haemolymph was performed for the identification of constituents and the extract revealed the presence of phenolic compounds, flavanoids, steroids, proreins and amino acids. In vitro anti-inflammatory activity was evaluated using HRBC membrane stabilisation method and protein denaturation method and confirmed that haemolymph extract showed anti-inflammatory activity. In vitro antiproliferative study of the haemolymph of Scylla serrata was evaluated by brine shrimp lethality assay, MTT assay and alamar blue assay in MCF-7 cells. The preliminary screening for anti mitotic activity of the extract was done in Allium cepa roots. Results indicated that the haemolymph of Scylla serrata possessed good antiproliferative property when compared to the standard drug doxorubicin