ANTIEPILEPTIC POTENTIAL AND MECHANISTIC EVALUATION OF PAVONIA PROCUMBENS: A STUDY ON FREE RADICAL SCAVENGING AND CHOLINESTERASE INHIBITION

The World Health Organization (WHO) describes traditional medicine, including herbal medications, as therapeutic modalities that predate the creation and dissemination of modern medicine and continue to be utilized today, often for hundreds of years. In this research, Pavonia procumbens was selected to investigate its antiepileptic potential and related mechanisms. The plant leaves were dried, powdered, and extracted using ethanol to produce the ethanol extract of Pavonia procumbens (EEPP), which was stored for further use. Various assays were employed to determine the extract's efficacy in scavenging free radicals through distinct reaction mechanisms. Additionally, the study explored the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities using Ellman’s method. Spectrophotometry-based assays utilizing specific substrates and a colorimetric method were conducted to measure AChE and BChE inhibition. The findings of this study contribute to understanding the therapeutic potential of Pavonia procumbens, particularly in its application as an antiepileptic agent, and support its relevance in traditional medicine practices