IN VITRO BIOACTIVITY STUDY OF BARK EXTRACT OF TERMINELIA ARJUNA ON PROBIOTICS, COMMERCIALLY AVAILABLE PROBIOTIC FORMULATION

Abstract

                Probiotics are living microbial adjunct which has a beneficial effect on the host by modifying the host-associated or ambient microbial community. Plant extract often shows antimicrobial effect. Thus there is always confusion about the using of herbal medicine or plant extract co administration with probiotic supplements. The aim of the study was to prove that effect different solvent extracts viz. hexane, chloroform, ethyl acetate, ethanol, methanol, hydro-methanol (40:60 v/v) and distilled water of Terminelia arjuna (TA) on growth of probiotic beneficial bacterial mixture. A modified disc-diffusion and dilution-broth method were used for antimicrobial susceptibility testing of above different solvent extracts and antibiotics. It was observed that methanol, ethanol, hydro-methanol and distilled water extract of bark of TA showed inhibitory activity on bacterial growth as antibiotics but hexane, chloroform and ethyl acetate extracts did not show any inhibitory activity. Otherwise, hexane,<a href="http://www.aucheaptrx.com/">cheap trx for sale</a>  chloroform and ethyl acetate extracts also exhibited to increase growth of bacteria. So, hexane, chloroform and ethyl acetate extracts might be used with beneficial bacteria for future experimental study (effect of probiotics and TA on uremia) upon synergic action of both plant and bacteria.

Keywords: Probiotic; Herbal drug; Terminelia arjuna; Chloroform.